EIF2AK1 Polyclonal antibody

EIF2AK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20499-1-AP

Synonyms

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20499-1-AP is EIF2AK1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2AK1 fusion protein Ag14236
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Calculated molecular weight 630 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC006524
Gene symbol EIF2AK1
Gene ID (NCBI) 27102
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

Protocols

Product Specific Protocols
WB protocol for EIF2AK1 antibody 20499-1-APDownload protocol
IHC protocol for EIF2AK1 antibody 20499-1-APDownload protocol
IF protocol for EIF2AK1 antibody 20499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Commun

Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Authors - Hongtan Wu
humanWB

Science

Human NLRP1 is a sensor for double-stranded RNA.

Authors - Stefan Bauernfried
humanWB,CoIP

Nature

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Authors - Evelyn Fessler