EIF3D Polyclonal antibody

EIF3D Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, yeast and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10219-1-AP

Synonyms

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7



Tested Applications

Positive WB detected inmouse brain tissue, A549 cells, HepG2 cells, human brain tissue, L02 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inN/A, transfected cells, MCF-7 cells, HepG2 cells, Ethacrynic acid treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10219-1-AP targets EIF3D in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, yeast samples.

Tested Reactivity human, mouse, yeast
Cited Reactivity human, mouse, rabbit, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3D fusion protein Ag0268
Full Name eukaryotic translation initiation factor 3, subunit D
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC000328
Gene symbol EIF3D
Gene ID (NCBI) 8664
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

Protocols

Product Specific Protocols
WB protocol for EIF3D antibody 10219-1-APDownload protocol
IHC protocol for EIF3D antibody 10219-1-APDownload protocol
IF protocol for EIF3D antibody 10219-1-APDownload protocol
IP protocol for EIF3D antibody 10219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

EBioMedicine

PMID: 31669222

Authors - Hai Huang
humanWB

Oncotarget

Proteomics analysis of bladder cancer invasion: Targeting EIF3D for therapeutic intervention.

Authors - Agnieszka Latosinska
humanIF

Cell Death Dis

Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.

Authors - Anaïs Aulas
rabbitWB

Methods Enzymol

A practical approach to isolate 48S complexes: affinity purification and analyses.

Authors - Locker Nicolas N
mouseIF

Biol Reprod

The LIF-mediated molecular signature regulating murine embryo implantation.

Authors - Gracy X Rosario
rabbitWB

RNA

Affinity purification of eukaryotic 48S initiation complexes.

Authors - Locker Nicolas N

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-10-2019)

EIF3D antibody recognizes a prominent band around the correct molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
EIF3D Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10219-1-AP)