|Positive WB detected in||mouse brain tissue, A549 cells, HepG2 cells, human brain tissue, L02 cells|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||N/A, transfected cells, MCF-7 cells, HepG2 cells, Ethacrynic acid treated HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10219-1-AP targets EIF3D in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, yeast samples.
|Tested Reactivity||human, mouse, yeast|
|Cited Reactivity||human, mouse, rabbit, yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF3D fusion protein Ag0268|
|Full Name||eukaryotic translation initiation factor 3, subunit D|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||BC000328|
|Gene ID (NCBI)||8664|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.
|Product Specific Protocols|
|WB protocol for EIF3D antibody 10219-1-AP||Download protocol|
|IHC protocol for EIF3D antibody 10219-1-AP||Download protocol|
|IF protocol for EIF3D antibody 10219-1-AP||Download protocol|
|IP protocol for EIF3D antibody 10219-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-10-2019)
EIF3D antibody recognizes a prominent band around the correct molecular weight.