EIF3D Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10219-1-AP
eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7
|Positive WB detected in||mouse brain tissue, A549 cells, HepG2 cells, human brain tissue, L02 cells|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||N/A, transfected cells, MCF-7 cells, HepG2 cells, Ethacrynic acid treated HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10219-1-AP targets EIF3D in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, yeast samples.
|Reactivity||human, mouse, yeast|
|Cited Species||human, mouse, rabbit, yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF3D fusion protein Ag0268|
|Full Name||eukaryotic translation initiation factor 3, subunit D|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||BC000328|
|Gene ID (NCBI)||8664|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.
|Product Specific Protocols|
|WB protocol for EIF3D antibody 10219-1-AP||Download protocol|
|IHC protocol for EIF3D antibody 10219-1-AP||Download protocol|
|IF protocol for EIF3D antibody 10219-1-AP||Download protocol|
|IP protocol for EIF3D antibody 10219-1-AP||Download protocol|
|Click here to view our Standard Protocols|
EIF3D promotes sunitinib resistance of renal cell carcinoma by interacting with GRP78 and inhibiting its degradation.
Proteomics analysis of bladder cancer invasion: Targeting EIF3D for therapeutic intervention.
Cell Death Dis
Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.
A practical approach to isolate 48S complexes: affinity purification and analyses.
The LIF-mediated molecular signature regulating murine embryo implantation.
Affinity purification of eukaryotic 48S initiation complexes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen Cheah (06-10-2019)
EIF3D antibody recognizes a prominent band around the correct molecular weight.