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EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11352-1-AP

Synonyms

eIF 3 p48, eIF3 p46, EIF3 P48, EIF3E, EIF3S6, INT6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EIF3E Polyclonal antibody (11352-1-AP)

    WB analysis of HeLa using 11352-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EIF3E Polyclonal antibody (11352-1-AP)

    WB analysis of HepG2 using 11352-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EIF3E Polyclonal antibody (11352-1-AP)

    WB analysis of HEK-293 using 11352-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using EIF3E Polyclonal antibody (11352-1-AP)

    WB analysis of Jurkat using 11352-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using EIF3E Polyclonal antibody (11352-1-AP)

    IP experiment of HepG2 using 11352-1-AP

    IP result of anti-EIF3E (IP:11352-1-AP, 4ug; Detection:11352-1-AP 1:500) with HepG2 cells lysate 880 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF3E Polyclonal antibody (11352-1-AP)

    IHC staining of human breast cancer using 11352-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF3E Polyclonal antibody (11352-1-AP)

    IHC staining of human breast cancer using 11352-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF3E Polyclonal antibody (11352-1-AP)

    IF Staining of HepG2 using 11352-1-AP

    Immunofluorescent analysis of HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF3E Polyclonal antibody (11352-1-AP)

    IF Staining of HepG2 using 11352-1-AP

    Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11352-1-AP targets EIF3E in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3E fusion protein Ag1856
Full Name eukaryotic translation initiation factor 3, subunit E
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberBC000734
Gene Symbol EIF3E
Gene ID (NCBI) 3646
RRIDAB_2277716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF3E antibody 11352-1-APDownload protocol
IHC protocol for EIF3E antibody 11352-1-APDownload protocol
IF protocol for EIF3E antibody 11352-1-APDownload protocol
IP protocol for EIF3E antibody 11352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols