EIF4A3 Polyclonal antibody

EIF4A3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 10463-1-AP

Synonyms

DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human lung tissue, mouse liver tissue, Raji cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

10463-1-AP targets EIF4A3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4A3 fusion protein Ag0733
Full Name eukaryotic translation initiation factor 4A, isoform 3
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC004386
Gene symbol EIF4A3
Gene ID (NCBI) 9775
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

Protocols

Product Specific Protocols
WB protocol for EIF4A3 antibody 10463-1-APDownload protocol
IP protocol for EIF4A3 antibody 10463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Signal

AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.

Authors - Izumi Natsuko N
humanWB,IP

Genes Dev

SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.

Authors - Yamashita Akio A