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ELP4 Polyclonal antibody

ELP4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12746-1-AP

Synonyms

C11orf19, Elongator complex protein 4, ELP4, hELP4, PAX6 neighbor gene protein, PAX6NEB, PAXNEB

Validation Data Gallery

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  • Western Blot (WB) analysis of human cerebellum tissue using ELP4 Polyclonal antibody (12746-1-AP)

    WB analysis of human cerebellum using 12746-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12746-1-AP (ELP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ELP4 Polyclonal antibody (12746-1-AP)

    WB analysis of human brain using 12746-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12746-1-AP (ELP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using ELP4 Polyclonal antibody (12746-1-AP)

    IHC staining of human endometrial cancer using 12746-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12746-1-AP (ELP4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using ELP4 Polyclonal antibody (12746-1-AP)

    IHC staining of human endometrial cancer using 12746-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12746-1-AP (ELP4 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inhuman cerebellum tissue, human brain tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12746-1-AP targets ELP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELP4 fusion protein Ag3707
Full Name elongation protein 4 homolog (S. cerevisiae)
Calculated Molecular Weight 424 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012514
Gene Symbol ELP4
Gene ID (NCBI) 26610
RRIDAB_2099745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ELP4 antibody 12746-1-APDownload protocol
IHC protocol for ELP4 antibody 12746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols