EML4 Antibody

EML4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22709-1-AP

Synonyms

C2orf2, ELP120, EMAP 4, EMAPL4, EML4, Ropp 120, ROPP120



Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 22709-1-AP immunogen is Fusion Protein EML4 protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EML4 fusion protein Ag18463
Full Name echinoderm microtubule associated protein like 4
Calculated molecular weight 981aa,109 kDa
Observed molecular weight 120 kDa
GenBank accession number BC140845
Gene symbol EML4
Gene ID (NCBI) 27436
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EML4 antibody 22709-1-APDownload protocol
IHC protocol for EML4 antibody 22709-1-APDownload protocol
IF protocol for EML4 antibody 22709-1-APDownload protocol
IP protocol for EML4 antibody 22709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols