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  • KD/KO Validated

EPHB4 Polyclonal antibody

EPHB4 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20883-1-AP

Synonyms

EPH receptor B4, EPHB4, Ephrin type B receptor 4, HTK, MYK1, TYRO11, Tyrosine protein kinase TYRO11



Tested Applications

Positive WB detected inrat testis tissue, DU 145 cells, HeLa cells, HT-1080 cells, PC-3 cells
Positive IHC detected inhuman placenta tissue, human lung cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20883-1-AP is EPHB4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPHB4 fusion protein Ag10033
Full Name EPH receptor B4
Calculated molecular weight 987 aa, 108 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC052804
Gene symbol EPHB4
Gene ID (NCBI) 2050
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EPHB4 antibody 20883-1-APDownload protocol
IHC protocol for EPHB4 antibody 20883-1-APDownload protocol
FC protocol for EPHB4 antibody 20883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Acta Neuropathol Commun

Low-level blast exposure disrupts gliovascular and neurovascular connections and induces a chronic vascular pathology in rat brain.

Authors - Miguel A Gama Sosa
humanIHC

Ann Vasc Surg

Adult Human Vein Grafts Retain Plasticity of Vessel Identity.

Authors - Hualong Bai
humanIHC

Odontology

Immunohistochemical assessment of Eph/ephrin expression in oral squamous cell carcinoma and precursor lesions.

Authors - Hiroki Saito
mouseWB

J Periodontal Res

Tumor necrosis factor-alpha inhibits osteogenic differentiation of pre-osteoblasts by downregulation of EphB4 signaling via activated nuclear factor-kappaB signaling pathway.

Authors - L M Wang
mouseWB

Cell Prolif

Low concentrations of TNF-α promote osteogenic differentiation via activation of the ephrinB2-EphB4 signalling pathway.

Authors - Limei Wang
  • KD Validated
mouseWB

Front Pharmacol

Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD.

Authors - Andrea Grandi