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EPO Polyclonal antibody

EPO Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17908-1-AP

Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2

Validation Data Gallery

  • WB analysis of HEK-293 using 17908-1-AP

    WB analysis of HEK-293 using 17908-1-AP

    WB result of EPO antibody (17908-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPO transfected HEK-293 cells.

  • IHC staining of human kidney using 17908-1-AP

    IHC staining of human kidney using 17908-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17908-1-AP (EPO Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 17908-1-AP

    IHC staining of human kidney using 17908-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17908-1-AP (EPO Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17908-1-AP is EPO Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPO fusion protein Ag12302
Full Name erythropoietin
Calculated molecular weight 193 aa, 21 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC093628
Gene symbol EPO
Gene ID (NCBI) 2056
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Protocols

Product Specific Protocols
WB protocol for EPO antibody 17908-1-APDownload protocol
IHC protocol for EPO antibody 17908-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols