EPRS Polyclonal antibody

EPRS Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25307-1-AP

Synonyms

EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25307-1-AP is EPRS Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPRS fusion protein Ag18011
Full Name glutamyl-prolyl-tRNA synthetase
Calculated molecular weight 1512 aa, 171 kDa
Observed molecular weight 171 kDa
GenBank accession numberBC126275
Gene symbol EPRS
Gene ID (NCBI) 2058
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)

Protocols

Product Specific Protocols
WB protocol for EPRS antibody 25307-1-APDownload protocol
IF protocol for EPRS antibody 25307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics

Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.

Authors - Jing Zhang