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WB analysis of K-562 using 12280-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12280-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12280-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12280-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12280-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12280-1-AP
IP result of anti-ETS2 (IP:12280-1-AP, 3ug; Detection:12280-1-AP 1:600) with HeLa cells lysate 5000ug.
IHC staining of mouse spleen using 12280-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 12280-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 12280-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 12280-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ETS2 Polyclonal antibody

Name: ETS2 Polyclonal antibody
Brand: Proteintech
SKU: 12280-1-AP

ETS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12280-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

ETS2, ETS2IT1, Protein C ets 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	K-562 cells, HeLa cells, MCF-7 cells, mouse kidney tissue, PC-3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse spleen tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

12280-1-AP targets ETS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2929 Product name: Recombinant human ETS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 84-444 aa of BC017040 Sequence: KATFSGFKKEQRRLGIPKNPWLWSEQQVCQWLLWATNEFSLVNVNLQRFGMNGQMLCNLGKERFLELAPDFVGDILWEHLEQMIKENQEKTEDQYEENSHLTSVPHWINSNTLGFGTEQAPYGMQTQNYPKGGLLDSMCPASTPSVLSSEQEFQMFPKSRLSSVSVTYCSVSQDFPGSNLNLLTNNSGTPKDHDSPENGADSFESSDSLLQSWNSQSSLLDVQRVPSFESFEDDCSQSLCLNKPTMSFKDYIQERSDPVEQGKPVIPAAVLAGFTGSGPIQLWQFLLELLSDKSCQSFISWTGDGWEFKLADPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTSGKRYVYRFVC Predict reactive species
Full Name	v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)
Calculated Molecular Weight	469 aa, 53 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC017040
Gene Symbol	ETS2
Gene ID (NCBI)	2114
RRID	AB_10804755
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15036
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ETS2 antibody 12280-1-AP	Download protocol
IP protocol for ETS2 antibody 12280-1-AP	Download protocol
WB protocol for ETS2 antibody 12280-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Death Dis The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation. Authors - Yan Li View Article
human	WB	Cell Death Dis A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer Authors - Yongheng Chen View Article
mouse	WB	Gene Cellular senescence-Related genes define the immune microenvironment and molecular characteristics in severe asthma patients Authors - Qibin Lin View Article
human	IHC	BMC Med Single-cell and spatial transcriptomics reveal metastasis mechanism and microenvironment remodeling of lymph node in osteosarcoma Authors - Yun Liu View Article