Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, MCF-7 cells, mouse kidney tissue, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse spleen tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12280-1-AP targets ETS2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2929 Product name: Recombinant human ETS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 84-444 aa of BC017040 Sequence: KATFSGFKKEQRRLGIPKNPWLWSEQQVCQWLLWATNEFSLVNVNLQRFGMNGQMLCNLGKERFLELAPDFVGDILWEHLEQMIKENQEKTEDQYEENSHLTSVPHWINSNTLGFGTEQAPYGMQTQNYPKGGLLDSMCPASTPSVLSSEQEFQMFPKSRLSSVSVTYCSVSQDFPGSNLNLLTNNSGTPKDHDSPENGADSFESSDSLLQSWNSQSSLLDVQRVPSFESFEDDCSQSLCLNKPTMSFKDYIQERSDPVEQGKPVIPAAVLAGFTGSGPIQLWQFLLELLSDKSCQSFISWTGDGWEFKLADPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTSGKRYVYRFVC Predict reactive species |
| Full Name | v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) |
| Calculated Molecular Weight | 469 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC017040 |
| Gene Symbol | ETS2 |
| Gene ID (NCBI) | 2114 |
| RRID | AB_10804755 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15036 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ETS2 antibody 12280-1-AP | Download protocol |
| IP protocol for ETS2 antibody 12280-1-AP | Download protocol |
| WB protocol for ETS2 antibody 12280-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ETS2 antibody (Cat# 12280-1-AP) has 9 citations spanning journals including Nature Microbiology, Cell Death & Disease, BMC Medicine, iScience, Frontiers in Pharmacology, Journal of Inflammation Research, Gene, and Ecotoxicology and Environmental Safety. Research fields cover innate antiviral immunity, colorectal cancer, osteosarcoma metastasis, ferroptosis in breast cancer, osteoarthritis, endothelial barrier integrity, asthma immune microenvironment, and air pollution-induced lung injury. Applications include WB, IP, IF, and IHC across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Nat Microbiol Oxaloacetate sensing promotes innate immune antiviral defence against influenza virus infection.
| ||
Cell Death Dis A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer | ||
Cell Death Dis The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation. | ||
BMC Med Single-cell and spatial transcriptomics reveal metastasis mechanism and microenvironment remodeling of lymph node in osteosarcoma | ||
Ecotoxicol Environ Saf Integrated toxicological analysis links air pollution-associated acute respiratory distress syndrome signatures to benzo[a]pyrene-induced acute lung injury. | ||
Front Pharmacol Magnolol triggers ferroptosis in triple-negative breast cancer through modulation of the ETS2/SLC7A11/GPX4 signaling axis. |
