Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83891-6-RR targets ETV4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0984 Product name: Recombinant human ETV4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC007242 Sequence: MYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPFGPKGGYSY Predict reactive species |
| Full Name | ets variant 4 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 61-65 kDa |
| GenBank Accession Number | BC007242 |
| Gene Symbol | ETV4 |
| Gene ID (NCBI) | 2118 |
| RRID | AB_3671474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43268 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ETV4 antibody 83891-6-RR | Download protocol |
| IHC protocol for ETV4 antibody 83891-6-RR | Download protocol |
| WB protocol for ETV4 antibody 83891-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ETV4 Recombinant monoclonal antibody (clone 241042E10), catalog number 83891-6-RR, has 1 citation. It appears in the FASEB Journal (2025), where the antibody was used for IHC and WB in human samples. The associated research field is oral squamous cell carcinoma metastasis, specifically investigating the Ca²⁺/Calmodulin/Calcineurin/ETV4 signaling pathway.
| Species | Application | Title |
|---|---|---|
FASEB J ORAI3 Modulates Oral Squamous Cell Carcinoma Metastasis Through the Ca(2+)/Calmodulin/Calcineurin/ETV4 Signaling Pathway. |
