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  • Featured Product
  • KD/KO Validated

EXOSC2 Polyclonal antibody

EXOSC2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14805-1-AP

Synonyms

EXOSC2, exosome component 2, hRrp4p, p7, RRP4, Rrp4p

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    WB analysis of HEK-293 using 14805-1-AP

    WB result of EXOSC2 antibody (14805-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    WB analysis of HeLa using 14805-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    WB analysis of HepG2 using 14805-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    WB analysis of Jurkat using 14805-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    WB analysis of HeLa using 14805-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    WB analysis of MCF-7 using 14805-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    WB analysis of HeLa using 14805-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    WB analysis of HepG2 using 14805-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    IP experiment of HeLa using 14805-1-AP

    IP result of anti-EXOSC2 (IP:14805-1-AP, 4ug; Detection:14805-1-AP 1:1000) with HeLa cells lysate 1080ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using EXOSC2 Polyclonal antibody (14805-1-AP)

    IHC staining of human skin cancer using 14805-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using EXOSC2 Polyclonal antibody (14805-1-AP)

    IHC staining of human skin cancer using 14805-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using EXOSC2 Polyclonal antibody (14805-1-AP)

    IF Staining of MCF-7 using 14805-1-AP

    Immunofluorescent analysis of MCF-7 cells, using EXOSC2 antibody 14805-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14805-1-AP targets EXOSC2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC2 fusion protein Ag6525
Full Name exosome component 2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC000747
Gene Symbol EXOSC2
Gene ID (NCBI) 23404
RRIDAB_2101837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].

Protocols

Product Specific Protocols
WB protocol for EXOSC2 antibody 14805-1-APDownload protocol
IHC protocol for EXOSC2 antibody 14805-1-APDownload protocol
IF protocol for EXOSC2 antibody 14805-1-APDownload protocol
IP protocol for EXOSC2 antibody 14805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell

Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.

Authors - Hani Goodarzi
View Article
human

Cell

The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.

Authors - Jakob Trendel
View Article
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
View Article
humanWB

Mol Biotechnol

EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal

Authors - Chen-Guang Lv
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tsimafei (Verified Customer) (12-04-2022)

50µg of total protein were loaded on a gel. Incubation ON at 4C

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mESCs
EXOSC2 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:14805-1-AP)
FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mESCs
EXOSC2 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:14805-1-AP)