Tested Applications
| Positive WB detected in | HEK-293T cells, Jurkat cells, HeLa cells, SW480 cells, HCT 116 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15937-1-AP targets EXOSC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7595 Product name: Recombinant human EXOSC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC002777 Sequence: MAGLELLSDQGYRVDGRRAGELRKIQARMGVFAQADGSAYIEQGNTKALAVVYGPHEIRGSRARALPDRALVNCQYSSATFSTGERKRRPHGDRKSCEMGLQLRQTFEAAILTQLHPRSQIDIYVQVLQADGGTYAACVNAATLAVLDAGIPMRDFVCACSAGFVDGTALADLSHVEEAAGGPQLALALLPASGQIALLEMDARLHEDHLERVLEAAAQAARDVHTLLDRVVRQHVREASILLGD Predict reactive species |
| Full Name | exosome component 4 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC002777 |
| Gene Symbol | EXOSC4 |
| Gene ID (NCBI) | 54512 |
| RRID | AB_2293806 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPD3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EXOSC4, also named as RRP41 and SKI6, belongs to the RNase PH family. EXOSC4 is a novel component of the human exosome complex. EXOSC4 is the most important RNA processing machinery in maturation and degradation of RNA playing a major role in gene expression regulation (PMID: 29626590). EXOSC4 has 1 isoform with the molecular mass of 27 kDa. However, studies have shown that EXOSC4 can interact with EXOSC2 and EXOSC7, possibly form a complex with the molecular weight of 90 kDa (PMID: 25115889). Recently, EXOSC4 has been found to play important roles in tumorigcncsis and development including CRC (PMID: 33075373).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EXOSC4 antibody 15937-1-AP | Download protocol |
| IHC protocol for EXOSC4 antibody 15937-1-AP | Download protocol |
| WB protocol for EXOSC4 antibody 15937-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-EXOSC4 (Cat# 15937-1-AP) has 6 total citations across journals including J Transl Med, EMBO J, Life Sci, J Biol Chem, Life Sci Alliance, and G3 (Bethesda). Research fields span RNA exosome structure and function, rheumatoid arthritis-associated interstitial lung disease, colorectal cancer proliferation, mRNA cap binding and translation, SARS-CoV-2 replication, and exosomopathy variant identification in yeast models.
| Species | Application | Title |
|---|---|---|
J Transl Med Identification EXOSC4 as a novel autoantigen of interstitial lung disease in rheumatoid arthritis | ||
Life Sci STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.
| ||
J Biol Chem A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. | ||
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication | ||
G3 (Bethesda) Humanized Saccharomyces cerevisiae provides a facile and effective tool to identify damaging human variants that cause exosomopathies |
Reviews
What customers say
Both reviewers gave this antibody a perfect 5-star rating for Western Blot applications. One user tested it on RKO cells at a 1:5000 dilution and described it as an excellent antibody against human Exosc4. The other validated it in mESCs at 1:2000 with overnight incubation at 4°C, confirming that Exosc4 depletion appropriately reduced the signal, demonstrating good specificity. Overall, users report strong performance and reliable results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aleksandr (Verified Customer) (02-07-2026) | Excellent antibody against human exosc4
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Tsimafei (Verified Customer) (07-06-2022) | Control and Exosc4 depletion shows a reduction of Exosc4 signal. Antibodies were incubated ON at 4C.
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