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- KD/KO Validated
EXOSC4 Polyclonal antibody
EXOSC4 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15937-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, Jurkat cells, HeLa cells, SW480 cells, HCT 116 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
15937-1-AP targets EXOSC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EXOSC4 fusion protein Ag7595 |
| Full Name | exosome component 4 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC002777 |
| Gene Symbol | EXOSC4 |
| Gene ID (NCBI) | 54512 |
| RRID | AB_2293806 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EXOSC4, also named as RRP41 and SKI6, belongs to the RNase PH family. EXOSC4 is a novel component of the human exosome complex. EXOSC4 is the most important RNA processing machinery in maturation and degradation of RNA playing a major role in gene expression regulation (PMID: 29626590). EXOSC4 has 1 isoform with the molecular mass of 27 kDa. However, studies have shown that EXOSC4 can interact with EXOSC2 and EXOSC7, possibly form a complex with the molecular weight of 90 kDa (PMID: 25115889). Recently, EXOSC4 has been found to play important roles in tumorigcncsis and development including CRC (PMID: 33075373).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOSC4 antibody 15937-1-AP | Download protocol |
| IHC protocol for EXOSC4 antibody 15937-1-AP | Download protocol |
| IF protocol for EXOSC4 antibody 15937-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. | ||
Life Sci STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.
| ||
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tsimafei (Verified Customer) (07-06-2022) | Control and Exosc4 depletion shows a reduction of Exosc4 signal. Antibodies were incubated ON at 4C.
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