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  • KD/KO Validated

EZH1 Polyclonal antibody

EZH1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20852-1-AP

Synonyms

Enhancer of zeste homolog 1, ENX 2, EZH1, KIAA0388



Tested Applications

Positive WB detected inRAW 264.7 cells, HL-60 cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20852-1-AP is EZH1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EZH1 fusion protein Ag14732
Full Name enhancer of zeste homolog 1 (Drosophila)
Calculated Molecular Weight 747 aa, 85 kDa
Observed Molecular Weight 80-95 kDa
GenBank Accession NumberBC015882
Gene Symbol EZH1
Gene ID (NCBI) 2145
RRIDAB_10863659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EZH1 antibody 20852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,chickenWB,IF

Nat Commun

Gain and loss of function variants in EZH1 disrupt neurogenesis and cause dominant and recessive neurodevelopmental disorders

Authors - Carolina Gracia-Diaz
mouseWB

Oxid Med Cell Longev

The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome.

Authors - Yichao Du
humanWB

Eur J Med Chem

Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.

Authors - Cheng Wang
mouseWB

Drug Discov Ther

Ezh1 regulates expression of Cpg15/Neuritin in mouse cortical neurons.

Authors - Shun Utsunomiya
  • KO Validated
mouseWB

Dev Cell

Polycomb Ezh1 maintains murine muscle stem cell quiescence through non-canonical regulation of Notch signaling

Authors - Xuesong Feng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mengxue (Verified Customer) (11-29-2018)

This is the 5th EZH1 Ab I've tried so far and the only one that works with our rat primary cells. I got clear band on Western and I'm so happy about it!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: rat primary fibroblast cells