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F11R Polyclonal antibody

F11R Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16183-1-AP

Synonyms

CD321, F11 receptor, F11R, JAM, JAM 1, JAM A, JAM1, JAMA, JAMA1, JCAM, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, PAM 1, Platelet adhesion molecule 1, Platelet F11 receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using F11R Polyclonal antibody (16183-1-AP)

    WB analysis of mouse liver using 16183-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16183-1-AP (F11R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using F11R Polyclonal antibody (16183-1-AP)

    WB analysis using 16183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16183-1-AP (F11R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using F11R Polyclonal antibody (16183-1-AP)

    IP experiment of mouse liver using 16183-1-AP

    IP result of anti-F11R (IP:16183-1-AP, 4ug; Detection:16183-1-AP 1:300) with mouse liver tissue lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using F11R Polyclonal antibody (16183-1-AP)

    IHC staining of human breast cancer using 16183-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16183-1-AP (F11R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using F11R Polyclonal antibody (16183-1-AP)

    IHC staining of human breast cancer using 16183-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16183-1-AP (F11R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, human peripheral blood platelets
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16183-1-AP targets F11R in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name F11 receptor
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001533
Gene Symbol F11R
Gene ID (NCBI) 50848
RRIDAB_2878226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and ligand for the integrin LFA1.

Protocols

Product Specific Protocols
WB protocol for F11R antibody 16183-1-APDownload protocol
IHC protocol for F11R antibody 16183-1-APDownload protocol
IP protocol for F11R antibody 16183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression.

Authors - Yongshuai Li
View Article
humanWB

Clin Exp Pharmacol Physiol

Rabeprazole destroyed gastric epithelial barrier function through FOXF1/STAT3-mediated ZO-1 expression

Authors - Fangying Yang
View Article
ratWB

J Chem Neuroanat

Matrine treatment induced an A2 astrocyte phenotype and protected the blood-brain barrier in CNS autoimmunity.

Authors - Yilei Jing
View Article