FA2H Polyclonal antibody

FA2H Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15452-1-AP


FA2H, FAAH, FAH1, fatty acid 2 hydroxylase, Fatty acid alpha hydroxylase, FAXDC1, SCS7

Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human stomach tissue, mouse colon tissue, MKN-45 cells, SGC-7901 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human colon cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15452-1-AP targets FA2H in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FA2H fusion protein Ag7717
Full Name fatty acid 2-hydroxylase
Calculated molecular weight 43 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberBC002679
Gene symbol FA2H
Gene ID (NCBI) 79152
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FA2H(Fatty acid 2-hydroxylase) required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. The deduced 372-amino acid protein has a calculated molecular mass of 42.8 kD .Both mouse and human FA2H have a C-terminal endoplasmic reticulum (ER) retention motif(PMID: 15658937).


Product Specific Protocols
WB protocol for FA2H antibody 15452-1-APDownload protocol
IHC protocol for FA2H antibody 15452-1-APDownload protocol
IF protocol for FA2H antibody 15452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Cell

Cell Lineage-Based Stratification for Glioblastoma.

Authors - Zilai Wang

Dev Cell

Quiescent human glioblastoma cancer stem cells drive tumor initiation, expansion, and recurrence following chemotherapy.

Authors - Xuanhua P Xie

Front Oncol

FA2H Exhibits Tumor Suppressive Roles on Breast Cancers via Cancer Stemness Control.

Authors - Xiaofeng Dai

J Med Chem

Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase.

Authors - Tyler D Baguley

Biochem Biophys Res Commun

Fatty acid 2-hydroxylase (FA2H) as a stimulatory molecule responsible for breast cancer cell migration.

Authors - Masayo Hirao-Suzuki

J Toxicol Sci

Perfluorooctanoic acid (PFOA) as a stimulator of estrogen receptor-negative breast cancer MDA-MB-231 cell aggressiveness: Evidence for involvement of fatty acid 2-hydroxylase (FA2H) in the stimulated cell migration.

Authors - Genki Sakai