FABP4 Monoclonal antibody

FABP4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E7E1

Cat no : 67167-1-Ig

Synonyms

A FABP, AFABP, ALBP, aP2, FABP4, Fatty acid binding protein 4



Tested Applications

Positive WB detected inrat heart tissue, mouse heart tissue, pig adipose tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:800-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67167-1-Ig targets FABP4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FABP4 fusion protein Ag8565
Full Name fatty acid binding protein 4, adipocyte
Calculated Molecular Weight 132 aa, 15 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC003672
Gene Symbol FABP4
Gene ID (NCBI) 2167
RRIDAB_2882463
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

Protocols

Product Specific Protocols
WB protocol for FABP4 antibody 67167-1-IgDownload protocol
IHC protocol for FABP4 antibody 67167-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Stem Cell Res Ther

Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.

Authors - Jie Zhou
mouseIHC

Int J Biol Sci

LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.

Authors - Yan Fang
humanWB

Front Oncol

Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.

Authors - Jinghui Lu
mouseWB

Biol Pharm Bull

Sorghum (Sorghum bicolor) Extract-Induced Adipogenesis Is Independent of PPARγ Ser273 Phosphorylation in 3T3-L1 Adipocytes.

Authors - Aozora Nagaoka