|Positive WB detected in||HT-1080 cells, HT-29 cells, Jurkat cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human prostate cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13098-1-AP targets FAS/CD95 in WB, IP, IHC, IF, FC, CHIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||hamster, human, monkey, sheep, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FAS/CD95 fusion protein Ag3718|
|Full Name||Fas (TNF receptor superfamily, member 6)|
|Calculated molecular weight||35-38 kDa|
|Observed molecular weight||38-45 kDa|
|GenBank accession number||BC012479|
|Gene ID (NCBI)||355|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediates apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.
|Product Specific Protocols|
|WB protocol for FAS/CD95 antibody 13098-1-AP||Download protocol|
|IHC protocol for FAS/CD95 antibody 13098-1-AP||Download protocol|
|IP protocol for FAS/CD95 antibody 13098-1-AP||Download protocol|
|FC protocol for FAS/CD95 antibody 13098-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kishor (Verified Customer) (06-23-2019)
This antibody works good at 1:1000 dilution in WB for mouse and human cells.