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FAS/CD95 Polyclonal antibody

FAS/CD95 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IP, IHC, IF, FC, CHIP, ELISA

Conjugate

Unconjugated

Cat no : 13098-1-AP

Synonyms

ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS1, FASLG receptor, FASTM, TNFRSF6



Tested Applications

Positive WB detected inHT-1080 cells, HT-29 cells, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13098-1-AP targets FAS/CD95 in WB, IP, IHC, IF, FC, CHIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity hamster, human, monkey, pig, sheep, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAS/CD95 fusion protein Ag3718
Full Name Fas (TNF receptor superfamily, member 6)
Calculated molecular weight 35-38 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC012479
Gene symbol FAS
Gene ID (NCBI) 355
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediates apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.

Protocols

Product Specific Protocols
WB protocol for FAS/CD95 antibody 13098-1-APDownload protocol
IHC protocol for FAS/CD95 antibody 13098-1-APDownload protocol
IP protocol for FAS/CD95 antibody 13098-1-APDownload protocol
FC protocol for FAS/CD95 antibody 13098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Physiol Biochem

α2,6-Sialylation promotes immune escape in hepatocarcinoma cells by regulating T cell functions and CD147/MMP signaling.

Authors - Liping Wang
mouseWB

Exp Ther Med

Effects of hypoxia ischemia on caspase-3 expression and neuronal apoptosis in the brain of neonatal mice.

Authors - Changbo Deng
swineWB

J Steroid Biochem Mol Biol

MicroRNA-128 is involved in dexamethasone-induced lipid accumulation via repressing SIRT1 expression in cultured pig preadipocytes.

Authors - Shifeng Pan
humanWB

Nanoscale Res Lett

Cytotoxicity induced by nanobacteria and nanohydroxyapatites in human choriocarcinoma cells.

Authors - Mingjun Zhang
mouseWB

Chronobiol Int

Maternal obesity disrupts circadian rhythms of clock and metabolic genes in the offspring heart and liver.

Authors - Danfeng Wang
humanWB

Asian Pac J Cancer Prev

Ganoderma lucidum polysaccharides target a fas/caspase dependent pathway to induce apoptosis in human colon cancer cells.

Authors - Zengenni Liang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kishor (Verified Customer) (06-23-2019)

This antibody works good at 1:1000 dilution in WB for mouse and human cells.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human and mouse cell lines
FAS/CD95 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in Human and mouse cell lines (Cat no:13098-1-AP)