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FBP1 Antibody

FBP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12842-1-AP

Synonyms

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1



Tested Applications

Positive WB detected inmouse liver tissue, human kidney tissue, human liver tissue, MCF-7 cells, rat liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

12842-1-AP targets FBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBP1 fusion protein Ag3837
Full Name fructose-1,6-bisphosphatase 1
Calculated molecular weight 338aa,37 kDa
Observed molecular weight 37 kDa
GenBank accession number BC012927
Gene symbol FBP1
Gene ID (NCBI) 2203
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBP1(Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD)(PMID:12126934).

Protocols

Product Specific Protocols
WB protocol for FBP1 antibody 12842-1-APDownload protocol
IHC protocol for FBP1 antibody 12842-1-APDownload protocol
IF protocol for FBP1 antibody 12842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Carcinogenesis

Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer.

Authors - Haoran Li
humanWB

Signal Transduct Target Ther

Hypoxia-induced GBE1 expression promotes tumor progression through metabolic reprogramming in lung adenocarcinoma.

Authors - Lifeng Li
humanWB

Pathol Oncol Res

The Promoting Effect of Radiation on Glucose Metabolism in Breast Cancer Cells under the Treatment of Cobalt Chloride.

Authors - Chun-Bo Zhao
pigWB

PLoS One

Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets.

Authors - Demin Cai
humanWB

Am J Cancer Res

HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.

Authors - Li-Na Yang