Tested Applications
| Positive WB detected in | A2780 cells, mouse ovary tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13024-1-AP targets FBX15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3564 Product name: Recombinant human FBXO15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC029579 Sequence: MPSEILLKIFSYLDAVSLLCTGCVSRRFYHLANDNFIWIGIYSTAFSPARSNWKFNSVEKIAMSMSFLSVQDKEAGYWKKEYITKQIASVKAALADILKPVNPYTGLPVKTKEALRIFGLGWAIILKEKGGKEYIMEHVDLSINDTSVTVIWYGKKWPCLASLSTLDLCGMTPVFTDWYKTPTKHRLRWHSLIAKYNLSHLTISTMIGCDRLIRIFCLHPGLLVGVWKKEEELAFVMANLHFHHLVERSTLGSATIPYELPPHSPFLDDSPEYGLHGYQLHVDLHSGGVFYLCGTFRNLFT Predict reactive species |
| Full Name | F-box protein 15 |
| Calculated Molecular Weight | 434 aa, 49 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC029579 |
| Gene Symbol | FBX15 |
| Gene ID (NCBI) | 201456 |
| RRID | AB_2104120 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NCQ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. The F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. FBOX15 (or FBX15) belongs to the FBXs class, and 2 isoforms produced by alternative splicing has been described. This antibody is expected detect both isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FBX15 antibody 13024-1-AP | Download protocol |
| WB protocol for FBX15 antibody 13024-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FBX15 Polyclonal antibody (Cat# 13024-1-AP) has 4 total citations. These appear in Phytomedicine, NPJ Precision Oncology, Clinical and Translational Medicine, and Scientific Reports. The research fields span Alzheimer's disease (P-glycoprotein ubiquitination), ovarian clear cell carcinoma (USP8/FGFR2 degradation), hepatocellular carcinoma (lipid metabolism reprogramming via FBXO11-mediated ubiquitination), and sepsis-related immunoparalysis (CHOP degradation via FBXO15). All citations used the antibody for Western blot in human or mouse samples.
| Species | Application | Title |
|---|---|---|
Phytomedicine Gastrodin reduces Aβ brain levels in an Alzheimer's disease mouse model by inhibiting P-glycoprotein ubiquitination | ||
NPJ Precis Oncol Targeting USP8 causes synthetic lethality through degradation of FGFR2 in ARID1A-deficient ovarian clear cell carcinoma | ||
Clin Transl Med Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma | ||
Sci Rep Oroxylin A alleviates immunoparalysis of CLP mice by degrading CHOP through interacting with FBXO15.
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