CD64 Antibody

CD64 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27563-1-AP

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, IGFR1, IgG Fc receptor I



Tested Applications

Positive WB detected inTHP-1 cells, U-937 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

27563-1-AP targets CD64 in WB, ELISA applications and shows reactivity with Human samples.

Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD64 fusion protein Ag26727
Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Calculated molecular weight 374aa,43 kDa
Observed molecular weight 65-70 kDa, 43-45 kDa
GenBank accession number BC032634
Gene symbol FCGR1A
Gene ID (NCBI) 2209
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.

Protocols

Product Specific Protocols
WB protocol for CD64 antibody 27563-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols