FCGR2B / CD32b Polyclonal antibody

FCGR2B / CD32b Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21541-1-AP

Synonyms

CD32, CD32B, CDw32, Fc gamma RII b, Fc gamma RIIb, FCG2, FCGR2, FCGR2B, FcRII b, IGFR2, IgG Fc receptor II b



Tested Applications

Positive WB detected inhuman placenta tissue, Raji cells
Positive IHC detected inhuman placenta tissue, human spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21541-1-AP is FCGR2B / CD32b Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCGR2B / CD32b fusion protein Ag16189
Full Name Fc fragment of IgG, low affinity IIb, receptor (CD32)
Calculated molecular weight 310 aa, 34 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC031992
Gene symbol FCGR2B
Gene ID (NCBI) 2213
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
IHC protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols