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- KD/KO Validated
Kindlin 2 Polyclonal antibody
Kindlin 2 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11453-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 12 publications below |
| WB | See 22 publications below |
| IHC | See 8 publications below |
| IF | See 8 publications below |
| IP | See 4 publications below |
Product Information
11453-1-AP targets Kindlin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Kindlin 2 fusion protein Ag2013 |
| Full Name | fermitin family homolog 2 (Drosophila) |
| Calculated Molecular Weight | 680 aa, 78 kDa |
| Observed Molecular Weight | 78 kDa |
| GenBank Accession Number | BC017327 |
| Gene Symbol | FERMT2 |
| Gene ID (NCBI) | 10979 |
| RRID | AB_2262660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| IHC protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| IP protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth. | ||
Circ Res Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.
| ||
J Am Soc Nephrol Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.
| ||
J Cell Sci Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.
| ||
J Cell Sci Kindlin-2 promotes rear focal adhesion disassembly and directional persistence in cell migration
| ||
Stem Cell Res Ther Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Yasmin (Verified Customer) (08-01-2019) | Excellent antibody, highly recommended for western blot.
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