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WB analysis of HeLa using 10966-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10966-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10966-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10966-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 10966-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10966-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10966-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 10966-1-AP
IP result of anti-FH (IP:10966-1-AP, 4ug; Detection:10966-1-AP 1:1000) with HepG2 cells lysate 2400ug.
IHC staining of human colon using 10966-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10966-1-AP (FH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 10966-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 10x lens).
IHC staining of human liver using 10966-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 40x lens).
IF Staining of HepG2 using 10966-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FH antibody (10966-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HepG2 using 10966-1-AP
Immunofluorescent analysis of HepG2 cells, using FH antibody 10966-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HepG2 using 10966-1-AP
Immunofluorescent analysis of HepG2 cells using 10966-1-AP (FH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

FH Polyclonal antibody

FH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10966-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:4.2.1.2, Fumarase, HsFH

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, HEK-293 cells, HepG2 cells, human liver tissue, L02 cells, mouse liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human colon tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

10966-1-AP targets FH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FH fusion protein Ag1437 Predict reactive species
Full Name	fumarate hydratase
Calculated Molecular Weight	55 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC017444
Gene Symbol	FH
Gene ID (NCBI)	2271
RRID	AB_568948
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07954
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts a tumor suppressor functions of FH in sporadic kidney cancer(PMID:21695080). This protein has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for FH antibody 10966-1-AP	Download protocol
IHC protocol for FH antibody 10966-1-AP	Download protocol
IF protocol for FH antibody 10966-1-AP	Download protocol
IP protocol for FH antibody 10966-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncotarget Identification of SPOP related metabolic pathways in prostate cancer. Authors - Min Yan View Article
human	IF	Endocr Relat Cancer Cellular kinetics of MED12-mutant uterine leiomyoma growth and regression in vivo. Authors - Vanida Ann Serna View Article
mouse,human	WB,IHC	Pharmaceuticals (Basel) Identification of a Difluorinated Alkoxy Sulfonyl Chloride as a Novel Antitumor Agent for Hepatocellular Carcinoma through Activating Fumarate Hydratase Activity Authors - Jin Jin View Article