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  • Featured Product
  • KD/KO Validated

FIGNL1 Polyclonal antibody

FIGNL1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17604-1-AP

Synonyms

fidgetin like 1, Fidgetin like protein 1, FIGNL1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FIGNL1 Polyclonal antibody (17604-1-AP)

    WB analysis using 17604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17604-1-AP (FIGNL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FIGNL1 Polyclonal antibody (17604-1-AP)

    IP experiment of HeLa using 17604-1-AP

    IP result of anti-FIGNL1 (IP:17604-1-AP, 4ug; Detection:17604-1-AP 1:1000) with HeLa cells lysate 2680ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FIGNL1 Polyclonal antibody (17604-1-AP)

    IF Staining of HeLa using 17604-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17604-1-AP (FIGNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HuH-7 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17604-1-AP targets FIGNL1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FIGNL1 fusion protein Ag11709
Full Name fidgetin-like 1
Calculated Molecular Weight 674 aa, 74 kDa
Observed Molecular Weight 66-71 kDa
GenBank Accession NumberBC051867
Gene Symbol FIGNL1
Gene ID (NCBI) 63979
RRIDAB_2104675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FIGNL1 antibody 17604-1-APDownload protocol
IF protocol for FIGNL1 antibody 17604-1-APDownload protocol
IP protocol for FIGNL1 antibody 17604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Cell Rep

C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair

Authors - Zenan Zhou
  • KD Validated
View Article
humanWB

Int J Oncol

FIGNL1 promotes non‑small cell lung cancer cell proliferation.

Authors - Miao Li
  • KD Validated
View Article
humanWB

Oncol Rep

PMID: 28260065

Authors - Jian Ma
  • KD Validated
View Article
humanWB,IP

bioRxiv

RADIF(C1orf112)-FIGNL1 Complex Regulates RAD51 Chromatin Association to Promote Viability After Replication Stress

Authors - Jessica D Tischler
View Article