Tested Applications
| Positive WB detected in | mouse spleen tissue, PC-3 cells, HeLa cells, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20712-1-AP targets FLT4/VEGFR3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, deer |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | fms-related tyrosine kinase 4 |
| Calculated Molecular Weight | 153 kDa |
| Observed Molecular Weight | 125 kDa |
| GenBank Accession Number | NM_002020 |
| Gene Symbol | FLT4 |
| Gene ID (NCBI) | 2324 |
| RRID | AB_10733474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FLT4, also named VEGFR-3, belongs to the protein kinase superfamily, Tyr protein kinase family, and CSF-1/PDGF receptor subfamily. It is a receptor for VEGFC. FLT4 has a tyrosine-protein kinase activity. Defects in FLT4 cause lymphedema hereditary type 1 (LYH1A). Defects in FLT4 are found in juvenile hemangioma. VEGFR-3 is a highly glycosylated protein and migrates as bands with different molecular weights: an ~175 kDa precursor, an ~195 kDa mature form, an ~140 kDa non-glycosylated backbone, and a form that appears to be partially cleaved proteolytically in the extracellular domain to produce an ~125 kDa band(PMID: 15389531).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FLT4/VEGFR3 antibody 20712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-VEGFR3 (FLT4) antibody, Cat# 20712-1-AP, has 20 citations published in journals including Molecular Cancer, International Journal of Biological Sciences, Stem Cell Research & Therapy, Acta Pharmacologica Sinica, ACS Pharmacology & Translational Science, Cell Signaling, and Frontiers in Oncology. Associated research fields encompass lymphangiogenesis, oncology (lung, pancreatic, thyroid, oral, hepatocellular cancers), obesity, spinal cord injury, cardiovascular disease, renal fibrosis, and corneal neovascularization.
| Species | Application | Title |
|---|---|---|
Mol Cancer m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma | ||
Int J Biol Sci PROTAC-Surufatinib Suppresses Pancreatic Neuroendocrine Neoplasms Progression by Inducing Ferroptosis through Inhibiting WNT/β-catenin Pathway Mediated by HMOX1 | ||
Phytomedicine Berberine promotes lacteal junction zippering and ameliorates diet-induced obesity through the RhoA/ROCK signaling pathway | ||
Acta Pharmacol Sin DsbA-L ameliorates renal aging and renal fibrosis by maintaining mitochondrial homeostasis | ||
Int J Biol Macromol Structural characterisation of a polysaccharide from Sanghuangporus vaninii and its synergistic anti-hepatocellular carcinoma activity in combination with sorafenib. | ||
Stem Cell Res Ther Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration. |
Reviews
What customers say
The single review for 20712-1-AP is from a user who tested it by Western Blot on lung cancer cell lines and tissues. They rated it 4 out of 5 stars. Using a 1:500 dilution, they reported obtaining a very strong band and noted that the product comes in a large volume, making it cost-effective for their needs.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shinford (Verified Customer) (12-15-2023) | We used the concentration of 1:500 to get a very strong band. It is a big volume set.
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