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FOXO3A Polyclonal antibody

FOXO3A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10849-1-AP

Synonyms

AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, PC-3 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10849-1-AP targets FOXO3A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXO3A fusion protein Ag1289
Full Name forkhead box O3
Calculated molecular weight 673 aa, 71 kDa
Observed molecular weight 72-97 kDa
GenBank accession numberBC021224
Gene symbol FOXO3
Gene ID (NCBI) 2309
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)

Protocols

Product Specific Protocols
WB protocol for FOXO3A antibody 10849-1-APDownload protocol
IHC protocol for FOXO3A antibody 10849-1-APDownload protocol
IF protocol for FOXO3A antibody 10849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

Theranostics

Caffeine Protects Skin from Oxidative Stress-Induced Senescence through the Activation of Autophagy.

Authors - Yi-Fang Li
humanWB,IHC

Mol Ther

PMID: 31153828

Authors - Yuting Sang

J Cancer Res Ther

Nuclear forkhead box O3a accumulation contributing to the proliferative suppression in liver cancer cells by PI3K/Akt signaling pathway.

Authors - Yibin Hou
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
humanWB,IF

Cell Death Dis

Chlorzoxazone, a small molecule drug, augments immunosuppressive capacity of mesenchymal stem cells via modulation of FOXO3 phosphorylation.

Authors - Luchan Deng
humanWB

Life Sci Alliance

CDKL3 promotes osteosarcoma progression by activating Akt/PKB.

Authors - Aina He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain