|Positive WB detected in||mouse heart tissue, mouse uterus tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21535-1-AP is FOXO4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FOXO4 fusion protein Ag16169|
|Full Name||forkhead box O4|
|Calculated molecular weight||505 aa, 54 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC106761|
|Gene ID (NCBI)||4303|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.
J Agric Food Chem
Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.
Onco Targets Ther
miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4.
FOXO4 expression associates with glioblastoma development and FOXO4 expression inhibits cell malignant phenotypes in vitro and in vivo.
Int J Biochem Cell Biol
Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway.
J Clin Invest
Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.
Suppression of Activated FOXO Transcription Factors in the Heart Prolongs Survival in a Mouse Model of Laminopathies.