Tested Applications
| Positive WB detected in | PC-12 cells, mouse heart tissue, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 18 publications below |
| IHC | See 5 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
21535-1-AP targets FOXO4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FOXO4 fusion protein Ag16169 Predict reactive species |
| Full Name | forkhead box O4 |
| Calculated Molecular Weight | 505 aa, 54 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC106761 |
| Gene Symbol | FOXO4 |
| Gene ID (NCBI) | 4303 |
| RRID | AB_10732960 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98177 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXO4 antibody 21535-1-AP | Download protocol |
| IHC protocol for FOXO4 antibody 21535-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Circ Res Suppression of Activated FOXO Transcription Factors in the Heart Prolongs Survival in a Mouse Model of Laminopathies. | ||
J Clin Invest Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.
| ||
Nat Commun Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors. | ||
Redox Biol Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction. | ||
Mater Today Bio Ultra-small polydopamine nanomedicine-enabled antioxidation against senescence | ||
J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH zhiping (Verified Customer) (06-04-2021) | It works pretty well for IHC.
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