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FOXO4 Polyclonal antibody

FOXO4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21535-1-AP

Synonyms

AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7



Tested Applications

Positive WB detected inmouse heart tissue, mouse uterus tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21535-1-AP is FOXO4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity DOG, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXO4 fusion protein Ag16169
Full Name forkhead box O4
Calculated molecular weight 505 aa, 54 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC106761
Gene symbol FOXO4
Gene ID (NCBI) 4303
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.

Protocols

Product Specific Protocols
WB protocol for FOXO4 antibody 21535-1-APDownload protocol
IHC protocol for FOXO4 antibody 21535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
humanIHC

Onco Targets Ther

miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4.

Authors - Shuai Chen
humanWB

Life Sci

FOXO4 expression associates with glioblastoma development and FOXO4 expression inhibits cell malignant phenotypes in vitro and in vivo.

Authors - Min Qi
mouseWB

Int J Biochem Cell Biol

Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway.

Authors - Yang Yang
humanWB

J Clin Invest

Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.

Authors - Xu Xiaojie X
  • KD Validated
DOGWB

Circ Res

Suppression of Activated FOXO Transcription Factors in the Heart Prolongs Survival in a Mouse Model of Laminopathies.

Authors - Gaelle Auguste