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Ferritin light chain Polyclonal antibody

Ferritin light chain Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10727-1-AP

Synonyms

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL



Tested Applications

Positive WB detected inmouse liver tissue, mouse ovary tissue, mouse brain tissue, mouse spleen tissue, human placenta tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10727-1-AP targets Ferritin light chain in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ferritin light chain fusion protein Ag1149
Full Name ferritin, light polypeptide
Calculated molecular weight 19 kDa
Observed molecular weight 20-22 kDa
GenBank accession numberBC008439
Gene symbol FTL
Gene ID (NCBI) 2512
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

Protocols

Product Specific Protocols
WB protocol for Ferritin light chain antibody 10727-1-APDownload protocol
IHC protocol for Ferritin light chain antibody 10727-1-APDownload protocol
IF protocol for Ferritin light chain antibody 10727-1-APDownload protocol
IP protocol for Ferritin light chain antibody 10727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice.

Authors - Meng-Wan Zhang
mouseWB

Cell Death Dis

Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload.

Authors - Junyan Wang
humanWB

Oncotarget

Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin.

Authors - Dong Xue
monkeyWB

Data Brief

Liver metal levels and expression of genes related to iron homeostasis in rhesus monkeys after inhalational manganese exposure.

Authors - Michael A Pettiglio
humanIHC

Syst Biol Reprod Med

Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.

Authors - Genhai Zhu
mouseWB

Neurosci Lett

Angiotensin II down-regulates transferrin receptor 1 and ferroportin 1 expression in Neuro-2a cells via activation of type-1 receptor.

Authors - Yun-Jin Chen