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Ferritin light chain Antibody 19 Publications

Rabbit Polyclonal| Catalog number: 10727-1-AP

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Con: 52 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Ferritin light chain Antibody WB mouse liver tissue 10727-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10727-1-AP (Ferritin light chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Ferritin light chain Antibody WB mouse liver tissue 10727-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 10727-1-AP(Ferritin light chain antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Ferritin light chain Antibody IP mouse liver tissue 10727-1-AP

IP Result of anti-Ferritin light chain (IP:10727-1-AP, 3ug; Detection:10727-1-AP 1:1000) with mouse liver tissue lysate 12500ug.

Ferritin light chain Antibody IHC human liver cancer tissue 10727-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10727-1-AP( Ferritin light chain antibody at dilution of 1:200 (under 10x lens).

Ferritin light chain Antibody IHC human liver cancer tissue 10727-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10727-1-AP( Ferritin light chain antibody at dilution of 1:200 (under 40x lens).

Ferritin light chain Antibody IF HepG2 cells 10727-1-AP

Immunofluorescent analysis of HepG2 cells using 10727-1-AP(Ferritin light chain antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, monkey
Positive WB detected in:
mouse liver tissue, mouse ovary tissue, mouse brain tissue, mouse spleen tissue, human placenta tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human liver cancer tissue, human liver tissue, human spleen tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:100-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Ferritin light chain fusion protein Ag1149
Full name:
ferritin, light polypeptide
Calculated molecular weight:
19 kDa
Observed molecular weight:
20-22 kDa
GenBank accession number:
BC008439
Gene ID (NCBI):
2512
Gene symbol
FTL
Synonyms
Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL

Background

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.


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