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Ferritin light chain Polyclonal antibody
Ferritin light chain Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10727-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, mouse ovary tissue, mouse brain tissue, mouse spleen tissue, human placenta tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver cancer tissue, human liver tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 21 publications below |
IHC | See 6 publications below |
IF | See 2 publications below |
Product Information
10727-1-AP targets Ferritin light chain in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, monkey, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Ferritin light chain fusion protein Ag1149 |
Full Name | ferritin, light polypeptide |
Calculated molecular weight | 19 kDa |
Observed molecular weight | 20-22 kDa |
GenBank accession number | BC008439 |
Gene symbol | FTL |
Gene ID (NCBI) | 2512 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.
Protocols
Product Specific Protocols | |
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WB protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
IHC protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
IF protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
IP protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging (Albany NY) Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice. | ||
Cell Death Dis Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload. | ||
Oncotarget Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin. | ||
Data Brief Liver metal levels and expression of genes related to iron homeostasis in rhesus monkeys after inhalational manganese exposure. | ||
Syst Biol Reprod Med Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts. | ||
Neurosci Lett Angiotensin II down-regulates transferrin receptor 1 and ferroportin 1 expression in Neuro-2a cells via activation of type-1 receptor. |