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- KD/KO Validated
Ferritin light chain Antibody
Ferritin light chain Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10727-1-AP
Synonyms
Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, mouse ovary tissue, mouse brain tissue, mouse spleen tissue, human placenta tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 19 publications below |
| IHC | See 6 publications below |
| IF | See 2 publications below |
Product Information
10727-1-AP targets Ferritin light chain in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Ferritin light chain fusion protein Ag1149 |
| Full Name | ferritin, light polypeptide |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 20-22 kDa |
| GenBank accession number | BC008439 |
| Gene symbol | FTL |
| Gene ID (NCBI) | 2512 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| IHC protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| IF protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| IP protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice. | ||
Cell Death Dis Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload. | ||
Oncotarget Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin. | ||
Data Brief Liver metal levels and expression of genes related to iron homeostasis in rhesus monkeys after inhalational manganese exposure. | ||
Syst Biol Reprod Med Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts. | ||
Neurosci Lett Angiotensin II down-regulates transferrin receptor 1 and ferroportin 1 expression in Neuro-2a cells via activation of type-1 receptor. |
