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FXR2 Polyclonal antibody

FXR2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12552-1-AP

Synonyms

FMR1L2, FXR2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FXR2 Polyclonal antibody (12552-1-AP)

    WB analysis of HeLa using 12552-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using FXR2 Polyclonal antibody (12552-1-AP)

    WB analysis of human brain using 12552-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FXR2 Polyclonal antibody (12552-1-AP)

    IP experiment of HeLa using 12552-1-AP

    IP result of anti-FXR2 (IP:12552-1-AP, 4ug; Detection:12552-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FXR2 Polyclonal antibody (12552-1-AP)

    IF Staining of HepG2 using 12552-1-AP

    Immunofluorescent analysis of HepG2 cells using 12552-1-AP (FXR2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHeLa cells, human brain tissue
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12552-1-AP targets FXR2 in WB, RIP, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXR2 fusion protein Ag3402
Full Name fragile X mental retardation, autosomal homolog 2
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC020090
Gene Symbol FXR2
Gene ID (NCBI) 9513
RRIDAB_2110720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FXR2 antibody 12552-1-APDownload protocol
IF protocol for FXR2 antibody 12552-1-APDownload protocol
IP protocol for FXR2 antibody 12552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanRIP

Elife

The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.

Authors - Belinda Baquero-Perez
View Article
humanWB

Proc Natl Acad Sci U S A

ARL6IP1 mediates small-molecule-induced alleviation of Alzheimer pathology through FXR1-dependent BACE1 translation initiation

Authors - Gui-Feng Zhou
  • KD Validated
View Article