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CL488-conjugated Fibronectin Antibody

Fibronectin Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1G10F9

Cat no : CL488-66042

Synonyms

CIG, Cold insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF



Tested Applications

Positive IF detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 66042-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The CL488-66042 immunogen is Fusion Protein CL488-conjugated Fibronectin protein expressed in E. coli.

Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fibronectin fusion protein Ag8016
Full Name fibronectin 1
Calculated molecular weight 2386aa,263 kDa
Observed molecular weight 263 kDa
GenBank accession number BC005858
Gene symbol FN1
Gene ID (NCBI) 2335
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20oC. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated Fibronectin antibody CL488-66042Download protocol
Standard Protocols
Click here to view our Standard Protocols