Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12410-1-AP targets GABRA1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3117 Product name: Recombinant human GABRA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-280 aa of BC030696 Sequence: LTGRSYGQPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVP Predict reactive species |
| Full Name | gamma-aminobutyric acid (GABA) A receptor, alpha 1 |
| Calculated Molecular Weight | 456 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC030696 |
| Gene Symbol | GABRA1 |
| Gene ID (NCBI) | 2554 |
| RRID | AB_2108692 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14867 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GABRA1 antibody 12410-1-AP | Download protocol |
| IP protocol for GABRA1 antibody 12410-1-AP | Download protocol |
| WB protocol for GABRA1 antibody 12410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GABRA1 Polyclonal antibody (Cat# 12410-1-AP) has 57 citations spanning journals including Nature Biotechnology, Nature Metabolism, Advanced Materials, Cell Reports, Molecular Psychiatry, Journal of Translational Medicine, CNS Neuroscience & Therapeutics, Biochemical Pharmacology, and eLife. Research fields cover GABAergic signaling, epilepsy, Alzheimer's and Parkinson's disease, sleep disorders, anxiety/depression, gut microbiome interactions, anesthesia, pain, autoimmune encephalitis, and acute lung injury.
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
Genes Dis The Traf2 and NcK interacting kinase inhibitor NCB-0846 suppresses seizure activity involving the decrease of GRIA1 | ||
Phytomedicine Baicalein alleviates anxiety symptom in Parkinson's disease by targeting Sema3A-mediated parvalbumin interneuron dysfunction. |
Reviews
What customers say
The single review for this GABRA1 antibody (used at 1:100 dilution for immunofluorescence on human brain cortex) is highly positive, rated 5 stars. The reviewer describes it as working perfectly and the best they have tried, noting clear punctate staining on the axons of GABAergic neurons in the upper cortical layers. Overall, the feedback highlights excellent specificity and performance for IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (11-05-2024) | This gabra1 antibody works perfectly, the best I have tried so far. It shows perfectly the puncta on the axons of gabaergic neurons on the upper layers of the cortex.
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