GABRA4 Polyclonal antibody

GABRA4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12979-1-AP

Synonyms

GABA A Receptor alpha 4, GABAA Receptor alpha 4, GABRA4



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse brain tissue, human kidney tissue, mouse kidney tissue
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12979-1-AP targets GABRA4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABRA4 fusion protein Ag3600
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 4
Calculated Molecular Weight 554 aa, 62 kDa
Observed Molecular Weight 62-70 kDa
GenBank Accession NumberBC035055
Gene Symbol GABRA4
Gene ID (NCBI) 2557
RRIDAB_2108996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABRA4, encoding the extrasynaptically located α4‐subunit of the GABAA receptor, is involved in the etiology of autism. (PMID: 32028044, 32033586)

Protocols

Product Specific Protocols
WB protocol for GABRA4 antibody 12979-1-APDownload protocol
IP protocol for GABRA4 antibody 12979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Electro-acupuncture alleviates adolescent cocaine exposure-enhanced anxiety-like behaviors in adult mice by attenuating the activities of PV interneurons in PrL.

Authors - Jiaxun Nie
humanIP,WB

Cell Mol Life Sci

Cyclin D1-Cdk4 regulates neuronal activity through phosphorylation of GABAA receptors

Authors - Neus Pedraza
humanWB

Genes Dis

The Traf2 and NcK interacting kinase inhibitor NCB-0846 suppresses seizure activity involving the decrease of GRIA1

Authors - Min Wang