GABRG2 Polyclonal antibody

GABRG2 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14104-1-AP

Synonyms

CAE2, ECA2, GABA A receptor gamma 2, GABAA receptor gamma 2, GABRG2, GEFSP3



Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inmouse cerebellum tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14104-1-AP targets GABRG2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABRG2 fusion protein Ag5237
Full Name gamma-aminobutyric acid (GABA) A receptor, gamma 2
Calculated Molecular Weight 467 aa, 54 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC059389
Gene Symbol GABRG2
Gene ID (NCBI) 2566
RRIDAB_10693527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.

Protocols

Product Specific Protocols
WB protocol for GABRG2 antibody 14104-1-APDownload protocol
IF protocol for GABRG2 antibody 14104-1-APDownload protocol
IP protocol for GABRG2 antibody 14104-1-APDownload protocol
FC protocol for GABRG2 antibody 14104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Transgenic overexpression of furin increases epileptic susceptibility.

Authors - Yi Yang
mouseWB,IP

CNS Neurosci Ther

STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons.

Authors - Juan Yang
ratIHC, IF

Neurosci Lett

Acupuncture Improves Locomotor Function by Enhancing GABA receptor Expression in Transient Focal Cerebral Ischemia Rats.

Authors - Qian Xu
mouseWB,IF

Neuropharmacology

A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine

Authors - Xiao-Hui Tang
mouseWB

Front Endocrinol (Lausanne)

Altered GnRH neuron and ovarian innervation characterize reproductive dysfunction linked to the Fragile X messenger ribonucleoprotein (Fmr1) gene mutation

Authors - Pedro A Villa
humanWB,IF

Nat Genet

Pan-cancer analysis identifies tumor-specific antigens derived from transposable elements

Authors - Nakul M Shah