Tested Applications
| Positive WB detected in | Neuro-2a cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF-P detected in | mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
Product Information
14104-1-AP targets GABRG2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GABRG2 fusion protein Ag5237 Predict reactive species |
| Full Name | gamma-aminobutyric acid (GABA) A receptor, gamma 2 |
| Calculated Molecular Weight | 467 aa, 54 kDa |
| Observed Molecular Weight | 54-60 kDa |
| GenBank Accession Number | BC059389 |
| Gene Symbol | GABRG2 |
| Gene ID (NCBI) | 2566 |
| RRID | AB_10693527 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18507 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GABRG2 antibody 14104-1-AP | Download protocol |
| IF protocol for GABRG2 antibody 14104-1-AP | Download protocol |
| IP protocol for GABRG2 antibody 14104-1-AP | Download protocol |
| FC protocol for GABRG2 antibody 14104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
CNS Neurosci Ther STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons. | ||
Neurosci Lett Acupuncture Improves Locomotor Function by Enhancing GABA receptor Expression in Transient Focal Cerebral Ischemia Rats. | ||
Neuropharmacology A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine | ||
Front Endocrinol (Lausanne) Altered GnRH neuron and ovarian innervation characterize reproductive dysfunction linked to the Fragile X messenger ribonucleoprotein (Fmr1) gene mutation | ||
Nat Genet Pan-cancer analysis identifies tumor-specific antigens derived from transposable elements |
