GAD65 Antibody

GAD65 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 20746-1-AP

Synonyms

GAD 65, GAD2, GAD65, Glutamate decarboxylase 2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20746-1-AP targets GAD65 in IF, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession number NM_000818
Gene symbol GAD2
Gene ID (NCBI) 2572
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and insulin secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

Protocols

Product Specific Protocols
WB protocol for GAD65 antibody 20746-1-APDownload protocol
IF protocol for GAD65 antibody 20746-1-APDownload protocol
IP protocol for GAD65 antibody 20746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Clin Sci (Lond)

Prenatal cold exposure causes hypertension in offspring by hyperactivity of the sympathetic nervous system.

Authors - Ken Chen
mouseWB

Mol Brain

The distinct role of NR2B subunit in the enhancement of visual plasticity in adulthood.

Authors - Hanxiao Liu
ratWB

Sci Rep

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Authors - Hsin-Hung Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana Yablonska (02-06-2020)

SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C Blocking: SEA Block Blocking Buffer 1h Primary Ab: O/N incubation at 4C, 1:1000 Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissues, human.
GAD65 Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human. (Cat no:20746-1-AP)