Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20746-1-AP targets GAD65 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | NM_000818 |
| Gene Symbol | GAD65 |
| Gene ID (NCBI) | 2572 |
| RRID | AB_10949196 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q05329 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for GAD65 antibody 20746-1-AP | Download protocol |
| WB protocol for GAD65 antibody 20746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-GABA antibody (Cat# 20746-1-AP) has 29 citations published in journals including Nature Cell Biology, Nature Communications, Journal of Experimental & Clinical Cancer Research, Advanced Science, Translational Psychiatry, Frontiers in Pharmacology, and BMC Biology. Associated research fields span neuroscience (GABAergic signaling, visual plasticity, depression, pain), oncology (brain metastasis, lung adenocarcinoma), metabolic disorders (obesity, gut–brain axis), hypertension, and immunology.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression. | ||
Nat Commun High-fat diet disrupts a septal control on feeding to promote obesity in male mice. | ||
J Exp Clin Cancer Res GABA regulates metabolic reprogramming to mediate the development of brain metastasis in non-small cell lung cancer | ||
Adv Sci (Weinh) Gold Nanostrip Array-Mediated Wireless Electrical Stimulation for Accelerating Functional Neuronal Differentiation. | ||
Research (Wash D C) The Hypothalamic Medial Preoptic Area-Paraventricular Nucleus Circuit Modulates Depressive-Like Behaviors in a Mouse Model of Postpartum Depression | ||
Transl Psychiatry A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota. |
Reviews
What customers say
Two reviews were found for this product. One user (2020) gave a 5-star rating for Western Blot on human striatal tissue at 1:1000 dilution, providing a detailed protocol with clean results. Another user (2024) gave a 2-star rating for immunofluorescence on human brain cortex at 1:50 dilution, reporting that the antibody failed to produce the expected cytoplasmic or puncta-like staining pattern in inhibitory neurons, showing only nuclear artifacts instead. Overall, performance appears application-dependent, with strong WB results but inconsistent IF performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (08-06-2024) | GAD2/GAD65 (green) is supposed to mark cytoplasmatically or puncta-like my inhibitory neurons, but this lot did not seem to work, as it seems only artifacts marking around the nuclei.
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Lana (Verified Customer) (02-06-2020) | SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.
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