GAGE1 Polyclonal antibody

GAGE1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12795-1-AP

Synonyms

Cancer/testis antigen 4.4, CT4.1, CT4.4, G antigen 1, G antigen 4, GAGE 4, GAGE1, GAGE4



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12795-1-AP targets GAGE1 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAGE1 fusion protein Ag3534
Full Name G antigen 1
Calculated Molecular Weight 139 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC036094
Gene Symbol GAGE1
Gene ID (NCBI) 2543
RRIDAB_2108363
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GAGE1 antibody 12795-1-APDownload protocol
IHC protocol for GAGE1 antibody 12795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols