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GAT1 Polyclonal antibody

GAT1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20298-1-AP

Synonyms

GABA Transporter 1, GABATHG, GABATR, GABT1, GAT 1, GAT1, SLC6A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAT1 Polyclonal antibody (20298-1-AP)

    WB analysis using 20298-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20298-1-AP (GAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GAT1 Polyclonal antibody (20298-1-AP)

    IHC staining of mouse cerebellum using 20298-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20298-1-AP (GAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GAT1 Polyclonal antibody (20298-1-AP)

    IHC staining of mouse cerebellum using 20298-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20298-1-AP (GAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNeuro-2a cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20298-1-AP targets GAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAT1 fusion protein Ag14109
Full Name solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 42 kDa, 67 kDa
GenBank Accession NumberBC033904
Gene Symbol SLC6A1
Gene ID (NCBI) 6529
RRIDAB_2878664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GAT1 antibody 20298-1-APDownload protocol
IHC protocol for GAT1 antibody 20298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols