GCK Antibody

GCK Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19666-1-AP

Synonyms

GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2



Tested Applications

Positive WB detected inrat liver tissue, mouse pancreas tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The 19666-1-AP immunogen is Fusion Protein GCK protein expressed in E. coli.

Tested Reactivity mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCK fusion protein Ag8116
Full Name glucokinase (hexokinase 4)
Calculated molecular weight 52 kDa
Observed molecular weight 50-52 kDa
GenBank accession number BC001890
Gene symbol GCK
Gene ID (NCBI) 2645
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.

Protocols

Product Specific Protocols
WB protocol for GCK antibody 19666-1-APDownload protocol
IHC protocol for GCK antibody 19666-1-APDownload protocol
IP protocol for GCK antibody 19666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Arthritis Res Ther

Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis.

Authors - Guanhua Song
ratWB

Biochem Biophys Res Commun

ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.

Authors - Kelei Dong
mouseWB

J Agric Food Chem

A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.

Authors - Cheng Liu
mouseWB

Sci Rep

Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy.

Authors - Mengyang Liu
humanWB

Toxicology

Retinol binding protein 4 mediates MEHP-induced glucometabolic abnormalities in HepG2 cells.

Authors - Fei Wang
ratWB

PLoS One

Efficacy of Berberine in Patients with Non-Alcoholic Fatty Liver Disease.

Authors - Hong-Mei Yan