Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16941-1-AP targets GFM2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10530 Product name: Recombinant human GFM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 430-779 aa of BC015712 Sequence: GNIALTVGLKHTATGDTIVSSKSSALAAARRAEREGEKKHRQNNEAERLLLAGVEIPEPVFFCTIEPPSLSKQPDLEHALKCLQREDPSLKVRLDPDSGQTVLCGMGELHIEIIHDRIKREYGLETYLGPLQVAYRETILNSVRATDTLDRTLGDKRHLVTVEVEARPIETSSVMPVIEFEYAESINEGLLKVSQEAIENGIHSACLQGPLLGSPIQDVAITLHSLTIHPGTSTTMISACVSRCVQKALKKADKQVLEPLMNLEVTVARDYLSPVLADLAQRRGNIQEIQTRQDNKVVIGFVPLAEIMGYSTVLRTLTSGSATFALELSTYQAMNPQDQNTLLNRRSGLT Predict reactive species |
| Full Name | G elongation factor, mitochondrial 2 |
| Calculated Molecular Weight | 779 aa, 87 kDa |
| Observed Molecular Weight | 87 kDa |
| GenBank Accession Number | BC015712 |
| Gene Symbol | GFM2 |
| Gene ID (NCBI) | 84340 |
| RRID | AB_2110151 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969S9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The GFM2 gene encodes a GTPase involved in mitochondrial protein synthesis. GFM2 mediates ribosome recycling but lacks translocation activity, whereas GFM1 catalyzes translocation during elongation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GFM2 antibody 16941-1-AP | Download protocol |
| IP protocol for GFM2 antibody 16941-1-AP | Download protocol |
| WB protocol for GFM2 antibody 16941-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GFM2 Polyclonal antibody (Cat# 16941-1-AP) has 4 total citations published in Nature Metabolism, EMBO Journal, Biochemical and Biophysical Research Communications, and Physiological Reports. The associated research fields include mitochondrial disease, liver cancer growth via mitochondrial translation, mitochondrial biogenesis, and skeletal muscle physiology in both human and mouse models.
| Species | Application | Title |
|---|---|---|
Nat Metab Tetracyclines promote survival and fitness in mitochondrial disease models.
| ||
EMBO J Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism | ||
Biochem Biophys Res Commun Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice. | ||
Physiol Rep Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. |
