|Positive WB detected in||Recombinant protein|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
HRP-66002 targets GFP tag in WB applications and shows reactivity with recombinant protein, aequorea victoria samples.
|Tested Reactivity||recombinant protein, aequorea victoria|
|Host / Isotype||Mouse / IgG2a|
|Full Name||GFP tag|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||-|
|GenBank accession number||U73901|
|Gene ID (NCBI)|
|Conjugate||HRP Fluorescent Dye|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYFP tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Aamir (Verified Customer) (01-08-2020)
Worked fine for WB