HRP-conjugated GFP tag Monoclonal antibody

GFP tag Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, aequorea victoria

Applications

WB, ELISA

Conjugate

HRP Fluorescent Dye

CloneNo.

1E10H7

Cat no : HRP-66002

Synonyms

CFP, eGFP, eYFP, GFP, GFP tag, HRP CFP, HRP eGFP, HRP GFP, HRP YFP, YFP



Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

HRP-66002 targets GFP tag in WB, ELISA applications and shows reactivity with recombinant protein, aequorea victoria samples.

Tested Reactivity recombinant protein, aequorea victoria
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name GFP tag
Calculated molecular weight 26 kDa
Observed molecular weight-
GenBank accession numberAVU73901
Gene symbol
Gene ID (NCBI)
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYFP tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP.


Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


Publications

SpeciesApplicationTitle

Plant Physiol

Upstream Open Reading Frame and Phosphate-Regulated Expression of Rice OsNLA1 Controls Phosphate Transport and Reproduction.

Authors - Shu-Yi Yang

Elife

A picorna-like virus suppresses the N-end rule pathway to inhibit apoptosis.

Authors - Zhaowei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Aamir (Verified Customer) (01-08-2020)

Worked fine for WB

  • Applications: Western Blot,
  • Primary Antibody Dilution: 10000
  • Cell Tissue Type: HEks