Tested Applications
| Positive WB detected in | HEK-293 cells, MKN-45 cells, T-47D cells, mouse colon tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12832-1-AP targets GIPC2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3566 Product name: Recombinant human GIPC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC036075 Sequence: MPLKLRGKKKAKSKETAGLVEGEPTGAGGGSLSASRAPARRLVFHAQLAHGSATGRVEGFSSIQELYAQIAGAFEISPSEILYCTLNTPKIDMERLLGGQLGLEDFIFAHVKGIEKEVNVYKSEDSLGLTITDNGVGYAFIKRIKDGGVIDSVKTICVGDHIESINGENIVGWRHYDVAKKLKELKKEELFTMKLIEPKKAFEIEPRSKAGKSSGEKIGCGRATLRLRSKGPATVEEMPSETKAKAIEKIDDVLELYMGIRDIDLATTMFEAGKDKVNPDEFAVALDETLGDFAFPDEFVFDVWGVIGDAKRRGL Predict reactive species |
| Full Name | GIPC PDZ domain containing family, member 2 |
| Calculated Molecular Weight | 315 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC036075 |
| Gene Symbol | GIPC2 |
| Gene ID (NCBI) | 54810 |
| RRID | AB_2109837 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TF65 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GIPC2 antibody 12832-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GIPC2 Polyclonal Antibody (Cat# 12832-1-AP) has 1 total citation. It appears in the journal Epigenomics (IF 3.1), published in 2023. The cited study investigated GIPC2 as a tumor suppressor gene in acute myeloid leukemia, demonstrating its role in inducing apoptosis of leukemia cells through regulation of the PI3K/AKT signaling pathway. The research field encompasses oncology, specifically leukemia biology and cell death mechanisms.
| Species | Application | Title |
|---|---|---|
Epigenomics GIPC2 is a tumor suppressor gene for acute myeloid leukemia and induces apoptosis of leukemia cells by regulating the PI3K/AKT pathway |
Reviews
What customers say
One reviewer used this antibody at a 1:10 dilution on human corneal endothelial cells for Western Blot and gave it a 5-star rating, noting that it produced a very good signal with specific and high-intensity bands, making it well-suited for straightforward Western blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zhiguo (Verified Customer) (08-10-2026) | Very good signal for simple western: specific and hight intensity
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