Connexin-43 Polyclonal antibody

Connexin-43 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15386-1-AP

Synonyms

Connexin-43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse heart tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 15386-1-AP is Connexin-43 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity calf, calves, cow, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Connexin-43 fusion protein Ag6698
Full Name gap junction protein, alpha 1, 43kDa
Calculated molecular weight 43 kDa
Observed molecular weight 43-47 kDa
GenBank accession numberBC026329
Gene symbol GJA1
Gene ID (NCBI) 2697
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

Publications

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Visualization of Annular Gap Junction Vesicle Processing: The Interplay Between Annular Gap Junctions and Mitochondria.

Authors - Cheryl L Bell
calfWB

J Appl Toxicol

Pharmacological inhibition of TLR4/NF-κB with TLR4-IN-C34 attenuated microcystin-leucine arginine toxicity in bovine Sertoli cells.

Authors - Elikanah Olusayo Adegoke
mouseIF

J Cell Sci

The N-cadherin interactome in primary cardiomyocytes as defined using quantitative proximity proteomics.

Authors - Yang Li
ratIF

Front Pharmacol

Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway.

Authors - Hongming Zhang
humanIF

Cancers (Basel)

Targeting the mDia Formin-Assembled Cytoskeleton Is an Effective Anti-Invasion Strategy in Adult High-Grade Glioma Patient-Derived Neurospheres.

Authors - Krista M Pettee
mouse

Perfusion

Bone marrow mesenchymal stem cells overexpressing GATA-4 improve cardiac function following myocardial infarction.

Authors - Ji-Gang He