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KD/KO Validated

KGA-Specific Polyclonal antibody

Name: KGA-Specific Polyclonal antibody
Brand: Proteintech
SKU: 20170-1-AP

KGA-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20170-1-AP

Synonyms

AAD20, GLS, GLS1, GLSK, glutaminase, K glutaminase, KGA, KGA-specific, KIAA0838, L glutamine amidohydrolase

Validation Data Gallery

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AllWBIPIHCIF

WB analysis of HEK-293 using 20170-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis using 20170-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 20170-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20170-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 20170-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 20170-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20170-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 20170-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20170-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 20170-1-AP
IP result of anti-KGA-Specific (IP:20170-1-AP, 3ug; Detection:20170-1-AP 1:800) with HEK-293 cells lysate 3200ug.
IHC staining of human kidney using 20170-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 20170-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens).
IHC staining of mouse kidney using 20170-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 20170-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 20170-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 20170-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HEK-293 using 20170-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 20170-1-AP (KGA-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 20170-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KGA-Specific antibody (20170-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 20170-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, mouse kidney tissue, human heart tissue, human brain tissue, K-562 cells, mouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human kidney tissue, human brain tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:20-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 1 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

20170-1-AP targets KGA-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KGA-Specific fusion protein Ag14002
Full Name	glutaminase
Calculated Molecular Weight	669 aa, 73 kDa
Observed Molecular Weight	66 kDa
GenBank Accession Number	BC038507
Gene Symbol	GLS
Gene ID (NCBI)	2744
RRID	AB_10665373
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.

Protocols

Product Specific Protocols
WB protocol for KGA-Specific antibody 20170-1-AP	Download protocol
IHC protocol for KGA-Specific antibody 20170-1-AP	Download protocol
IF protocol for KGA-Specific antibody 20170-1-AP	Download protocol
IP protocol for KGA-Specific antibody 20170-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Med Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia. Authors - Daniel Herranz View Article
mouse	WB	Cell Stem Cell CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion. Authors - Gianluca L Russo View Article
human	WB	Blood Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition. Authors - Nathalie Jacque View Article
rat	CoIP, WB	Int J Mol Sci Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase. Authors - Małgorzata Beręsewicz-Haller View Article
human	WB,IHC	EBioMedicine Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway. Authors - Binghua Li View Article
human	WB	J Genet Genomics A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues. Authors - Yicheng Chen View Article

KGA-Specific Polyclonal antibody

KGA-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 20170-1-AP

WB analysis using 20170-1-AP

WB analysis using 20170-1-AP

WB analysis of HEK-293 using 20170-1-AP

WB analysis of mouse kidney using 20170-1-AP

WB analysis of human heart using 20170-1-AP

WB analysis of human brain using 20170-1-AP

WB analysis of K-562 using 20170-1-AP

WB analysis of mouse brain using 20170-1-AP

IP experiment of HEK-293 using 20170-1-AP

IHC staining of human kidney using 20170-1-AP

IHC staining of human kidney using 20170-1-AP

IHC staining of mouse kidney using 20170-1-AP

IHC staining of mouse kidney using 20170-1-AP

IHC staining of human brain using 20170-1-AP

IHC staining of human brain using 20170-1-AP

IF Staining of HEK-293 using 20170-1-AP

IF Staining of HEK-293 using 20170-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia.

CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.

Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.

Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues.