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GMPPA Polyclonal antibody

GMPPA Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15517-1-AP

Synonyms

GMPPA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GMPPA Polyclonal antibody (15517-1-AP)

    WB analysis using 15517-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15517-1-AP (GMPPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using GMPPA Polyclonal antibody (15517-1-AP)

    IP experiment of HEK-293 using 15517-1-AP

    IP result of anti-GMPPA (IP:15517-1-AP, 4ug; Detection:15517-1-AP 1:500) with HEK-293 cells lysate 2600ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GMPPA Polyclonal antibody (15517-1-AP)

    IF Staining of HeLa using 15517-1-AP

    Immunofluorescent analysis of Hela cells, using GMPPA antibody 15517-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15517-1-AP targets GMPPA in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMPPA fusion protein Ag7789
Full Name GDP-mannose pyrophosphorylase A
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC007456
Gene Symbol GMPPA
Gene ID (NCBI) 29926
RRIDAB_2111056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GMPPA antibody 15517-1-APDownload protocol
IF protocol for GMPPA antibody 15517-1-APDownload protocol
IP protocol for GMPPA antibody 15517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.

Authors - Norio Sasaoka
View Article
mouseWB

Front Mol Biosci

Altered Glycosylation in the Aging Heart.

Authors - Patricia Franzka
View Article
human,mouseWB

Front Nutr

Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12

Authors - Patricia Franzka
View Article
mouseWB

Biomedicines

Impact of Hypermannosylation on the Structure and Functionality of the ER and the Golgi Complex

Authors - Patricia Franzka
  • KO Validated
View Article