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  • KD/KO Validated

GNL3 Polyclonal antibody

GNL3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15060-1-AP

Synonyms

C77032, E2 induced gene 3 protein, E2IG3, GNL3, NNP47, Novel nucleolar protein 47, NS, Nucleostemin



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, HeLa cells, Raji cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15060-1-AP targets GNL3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNL3 fusion protein Ag7056
Full Name guanine nucleotide binding protein-like 3 (nucleolar)
Calculated molecular weight 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC001024
Gene symbol GNL3
Gene ID (NCBI) 26354
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GNL3 antibody 15060-1-APDownload protocol
IHC protocol for GNL3 antibody 15060-1-APDownload protocol
IF protocol for GNL3 antibody 15060-1-APDownload protocol
IP protocol for GNL3 antibody 15060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Manag Res

The oncogenic role of GNL3 in the progression and metastasis of osteosarcoma.

Authors - Tianyou Li
  • KD Validated
humanWB

J Nanobiotechnology

Targeted micelles with chemotherapeutics and gene drugs to inhibit the G1/S and G2/M mitotic cycle of prostate cancer.

Authors - Yiran Zhang