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GORASP2 Antibody 24 Publications

Rabbit Polyclonal| Catalog number: 10598-1-AP

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Con: 29 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

GORASP2 Antibody WB HeLa cells 10598-1-AP

WB result of GORASP2 antibody (10598-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GORASP2 transfected HeLa cells

GORASP2 Antibody WB COLO 320 cells 10598-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

GORASP2 Antibody WB HeLa cells 10598-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 10598-1-AP(GORASP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

GORASP2 Antibody IHC human colon cancer tissue 10598-1-AP

Immunohistochemical of paraffin-embedded human colon cancer using 10598-1-AP(GORASP2 antibody) at dilution of 1:100 (under 10x lens)

GORASP2 Antibody IP HeLa cells 10598-1-AP

IP Result of anti-GORASP2 (IP:10598-1-AP, 3ug; Detection:10598-1-AP 1:700) with HeLa cells lysate 1320ug.

GORASP2 Antibody IF HepG2 cells 10598-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 10598-1-AP(GORASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, monkey
Positive WB detected in:
HeLa cells, rat testis tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GORASP2 fusion protein Ag0950
Full name:
golgi reassembly stacking protein 2, 55kDa
Calculated molecular weight:
55 kDa
Observed molecular weight:
59 kDa
GenBank accession number:
BC007770
Gene ID (NCBI):
26003
Gene symbol
GORASP2
Synonyms
Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59

Background

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.


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