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  • KD/KO Validated

GORASP2 Antibody

GORASP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10598-1-AP

Synonyms

Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59



Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, rat testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10598-1-AP targets GORASP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GORASP2 fusion protein Ag0950
Full Name golgi reassembly stacking protein 2, 55kDa
Calculated molecular weight 55 kDa
Observed molecular weight 59 kDa
GenBank accession number BC007770
Gene symbol GORASP2
Gene ID (NCBI) 26003
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Protocols

Product Specific Protocols
WB protocol for GORASP2 antibody 10598-1-APDownload protocol
IHC protocol for GORASP2 antibody 10598-1-APDownload protocol
IF protocol for GORASP2 antibody 10598-1-APDownload protocol
IP protocol for GORASP2 antibody 10598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Dev Cell

A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport.

Authors - Mafalda Lopes-da-Silva
humanWB, IF

Biochem Biophys Res Commun

CD83 and GRASP55 interact in human dendritic cells.

Authors - Marcello F Stein
humanWB,IF

Cell Signal

DRAM1 deficiency affects the organization and function of the Golgi apparatus.

Authors - Mingzhen Wei
humanWB

Mol Biol Cell

DjA1 maintains Golgi integrity via interaction with GRASP65.

Authors - Jie Li
humanWB,IF

Mol Biol Cell

Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.

Authors - Michael E Bekier
  • KO Validated
humanWB

Mol Biol Cell

Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.

Authors - Stephen C Ireland

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Uxoa (Verified Customer) (02-18-2020)

GORASP2 (alexa fluor 488, green), Vimentin (alexa fluor 647, white), DAPI (blue). Primary fibroblasts fixed with 4% PFA 15min. 1:100 GORASP2 ab dilution, in PBST (0.1% triton) + 10% DS O/N 4ºC.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Primary human fibroblasts
GORASP2 Antibody Immunofluorescence, validation (1:100 dilution) in Primary human fibroblasts (Cat no:10598-1-AP)