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- KD/KO Validated
GORASP2 Polyclonal antibody
GORASP2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 10598-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, COLO 320 cells, rat testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 15 publications below |
| WB | See 23 publications below |
| IHC | See 2 publications below |
| IF | See 13 publications below |
Product Information
10598-1-AP targets GORASP2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GORASP2 fusion protein Ag0950 |
| Full Name | golgi reassembly stacking protein 2, 55kDa |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC007770 |
| Gene symbol | GORASP2 |
| Gene ID (NCBI) | 26003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| IHC protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| IF protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| IP protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport. | ||
Int J Mol Sci Mutations in GDAP1 Influence Structure and Function of the Trans-Golgi Network. | ||
Cell Signal DRAM1 deficiency affects the organization and function of the Golgi apparatus. | ||
Mol Biol Cell Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ana (Verified Customer) (03-14-2021) | GORASP, golgi labeling in human primary fibroblasts. PFA 4% fixed cells. 1:100 primary ab in 5% GS PBST (0.1% triton) O/N 4ºC incubation. 1:500 secondary ab Alexafluor 488 1h RT.
 |
FH Uxoa (Verified Customer) (02-18-2020) | GORASP2 (alexa fluor 488, green), Vimentin (alexa fluor 647, white), DAPI (blue). Primary fibroblasts fixed with 4% PFA 15min. 1:100 GORASP2 ab dilution, in PBST (0.1% triton) + 10% DS O/N 4ºC.
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