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WB analysis of Jurkat using 12095-1-AP
WB result of GOSR2/Membrin antibody (12095-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.
WB analysis using 12095-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12095-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12095-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse stomach using 12095-1-AP
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 12095-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12095-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOSR2/Membrin antibody (12095-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 12095-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 12095-1-AP
Immunofluorescent analysis of () fixed HepG2 cells using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Featured Product
KD/KO Validated

GOSR2/Membrin Polyclonal antibody

Name: GOSR2/Membrin Polyclonal antibody
Brand: Proteintech
SKU: 12095-1-AP

GOSR2/Membrin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12095-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Membrin, GS27, GOSR2, Golgi SNAP receptor complex member 2, 27 kDa Golgi SNARE protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, human liver tissue, mouse liver tissue, mouse stomach tissue, L02 cells, rat liver tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

12095-1-AP targets GOSR2/Membrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2736 Product name: Recombinant human GOSR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-192 aa of BC009710 Sequence: MDPLFQQTHKQVHEIQSCMGRLETADKQSVHIVENEIQASIDQIFSRLERLEILSSKEPPNKRQNARLRVDQLKYDVQHLQTALRNFQHRRHAREQQERQREELLSRTFTTNDSDTTIPMDESLQFNSSLQKVHNGMDDLILDGHNILDGLRTQRLTLKGTQKKILDIANMLGLSNTVMRLIEKRAFQDKYF Predict reactive species
Full Name	golgi SNAP receptor complex member 2
Calculated Molecular Weight	212 aa, 25 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC009710
Gene Symbol	Membrin
Gene ID (NCBI)	9570
RRID	AB_2113610
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14653
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

Protocols

Product Specific Protocols
IF protocol for GOSR2/Membrin antibody 12095-1-AP	Download protocol
IHC protocol for GOSR2/Membrin antibody 12095-1-AP	Download protocol
WB protocol for GOSR2/Membrin antibody 12095-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models Authors - Ying Xue Xie View Article
	WB	Commun Biol Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. Authors - Xihua Yue View Article