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Tested Applications
| Positive WB detected in | Human peripheral blood platelets, pig peripheral blood leukocyte, human blood |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IHC | See 12 publications below |
| IF | See 3 publications below |
Product Information
12860-1-AP targets CD42b in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD42b fusion protein Ag3893 Predict reactive species |
| Full Name | glycoprotein Ib (platelet), alpha polypeptide |
| Calculated Molecular Weight | 626 aa, 69 kDa |
| Observed Molecular Weight | 145 kDa |
| GenBank Accession Number | BC027955 |
| Gene Symbol | CD42b |
| Gene ID (NCBI) | 2811 |
| ENSEMBL Gene ID | ENSG00000185245 |
| RRID | AB_10644481 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07359 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GP1BA, also named as CD42b , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD42b antibody 12860-1-AP | Download protocol |
| IHC protocol for CD42b antibody 12860-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Platelet Membrane-Fused Circulating Extracellular Vesicles Protect the Heart from Ischemia/Reperfusion Injury | ||
Cancer Lett Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma | ||
Theranostics A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance. | ||
Int J Biol Macromol Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing | ||
ACS Biomater Sci Eng Biomimetic Platform Based on Mesoporous Platinum for Multisynergistic Cancer Therapy. | ||
Biochem Pharmacol GSK669, a NOD2 receptor antagonist, inhibits thrombosis and oxidative stress via targeting platelet GPVI. |
