CD42b Polyclonal antibody

CD42b Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12860-1-AP

Synonyms

BSS, CD42b, CD42b alpha, GP1B, GP1BA



Tested Applications

Positive WB detected inHuman peripheral blood platelets, pig peripheral blood leukocyte, human blood
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12860-1-AP targets CD42b in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD42b fusion protein Ag3893
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated molecular weight 626 aa, 69 kDa
Observed molecular weight 145 kDa
GenBank accession numberBC027955
Gene symbol GP1BA
Gene ID (NCBI) 2811
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GP1BA, also named as CD42b , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

Protocols

Product Specific Protocols
WB protocol for CD42b antibody 12860-1-APDownload protocol
IHC protocol for CD42b antibody 12860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIHC

Adv Exp Med Biol

Protective Effect of Taurine on Apoptosis of Spinal Cord Cells in Diabetic Neuropathy Rats.

Authors - Xiaoxia Inam-U-Llah
humanIHC

Placenta

Adhering maternal platelets can contribute to the cytokine and chemokine cocktail released by human first trimester villous placenta.

Authors - A Blaschitz
human,mouseIHC

Immunol Res

Von Willebrand factor protects against acute CCl4-induced hepatotoxicity through phospho-p38 MAPK signaling pathway inhibition.

Authors - Hai-Jian Sun
humanIHC

Hum Pathol

Transforming growth factor β- and interleukin 13-producing mast cells are associated with fibrosis in bone marrow.

Authors - Shoko Nakayama
rat, mouseWB

Cell Biosci

Hydrogen sulfide decreases high glucose/palmitate-induced autophagy in endothelial cells by the Nrf2-ROS-AMPK signaling pathway.

Authors - Jiaqi Liu
humanIHC

Lab Invest

TNF-α alters the inflammatory secretion profile of human first trimester placenta.

Authors - Monika Siwetz