CD42b Polyclonal antibody
CD42b Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, pig and More (2)
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
Cat no : 12860-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Human peripheral blood platelets, pig peripheral blood leukocyte, human blood |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IHC | See 6 publications below |
Product Information
12860-1-AP targets CD42b in WB, IHC,ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, pig, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD42b fusion protein Ag3893 |
| Full Name | glycoprotein Ib (platelet), alpha polypeptide |
| Calculated molecular weight | 626 aa, 69 kDa |
| Observed molecular weight | 145 kDa |
| GenBank accession number | BC027955 |
| Gene symbol | GP1BA |
| Gene ID (NCBI) | 2811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GP1BA, also named as CD42b , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD42b antibody 12860-1-AP | Download protocol |
| IHC protocol for CD42b antibody 12860-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Exp Med Biol Protective Effect of Taurine on Apoptosis of Spinal Cord Cells in Diabetic Neuropathy Rats. | ||
Placenta Adhering maternal platelets can contribute to the cytokine and chemokine cocktail released by human first trimester villous placenta. | ||
Immunol Res Von Willebrand factor protects against acute CCl4-induced hepatotoxicity through phospho-p38 MAPK signaling pathway inhibition. | ||
Chin Med Anti-platelet aggregation of Panax notoginseng triol saponins by regulating GP1BA for ischemic stroke therapy. | ||
Hum Pathol Transforming growth factor β- and interleukin 13-producing mast cells are associated with fibrosis in bone marrow. | ||
Cell Biosci Hydrogen sulfide decreases high glucose/palmitate-induced autophagy in endothelial cells by the Nrf2-ROS-AMPK signaling pathway. |
