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CD42a / GP9 Monoclonal antibody

CD42a / GP9 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D4H1

Cat no : 67271-1-Ig

Synonyms

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CD42a / GP9 Monoclonal antibody (67271-1-Ig)

    WB analysis of human peripheral blood platelets using 67271-1-Ig

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD42a / GP9 Monoclonal antibody (67271-1-Ig)

    IHC staining of human spleen using 67271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD42a / GP9 Monoclonal antibody (67271-1-Ig)

    IHC staining of human spleen using 67271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHuman peripheral blood platelets tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67271-1-Ig targets CD42a / GP9 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD42a / GP9 fusion protein Ag6132
Full Name glycoprotein IX (platelet)
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC030229
Gene Symbol GP9
Gene ID (NCBI) 2815
RRIDAB_2882540
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.

Protocols

Product Specific Protocols
WB protocol for CD42a / GP9 antibody 67271-1-IgDownload protocol
IHC protocol for CD42a / GP9 antibody 67271-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols