GP9 Monoclonal antibody

GP9 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D4H1

Cat no : 67271-1-Ig

Synonyms

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX



Tested Applications

Positive WB detected inHuman peripheral blood platelets tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67271-1-Ig targets GP9 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GP9 fusion protein Ag6132
Full Name glycoprotein IX (platelet)
Calculated molecular weight 19 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC030229
Gene symbol GP9
Gene ID (NCBI) 2815
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.

Protocols

Product Specific Protocols
WB protocol for GP9 antibody 67271-1-IgDownload protocol
IHC protocol for GP9 antibody 67271-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols