GRIA4 Polyclonal antibody

GRIA4 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 23350-1-AP

Synonyms

GluA4, GluR 4, GluR D, GLUR4, GLUR4C, GLURD, Glutamate receptor 4, GRIA4



Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue, mouse cerebellum tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23350-1-AP is GRIA4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRIA4 fusion protein Ag19949
Full Name glutamate receptor, ionotrophic, AMPA 4
Calculated molecular weight 902 aa, 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC167786
Gene symbol GRIA4
Gene ID (NCBI) 2893
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRIA4 antibody 23350-1-APDownload protocol
IHC protocol for GRIA4 antibody 23350-1-APDownload protocol
IP protocol for GRIA4 antibody 23350-1-APDownload protocol
FC protocol for GRIA4 antibody 23350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochim Biophys Acta Mol Cell Res

Behavioral and electrophysiological changes in female mice overexpressing ORAI1 in neurons.

Authors - Filip Maciąg
humanWB

Front Cell Dev Biol

Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models.

Authors - Shirong Tang
mouseIP

Cell Death Dis

ZDHHC8 critically regulates seizure susceptibility in epilepsy.

Authors - Qin Yang
humanWB

J Clin Invest

TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.

Authors - Haiqing Zhang